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smc maintaining media  (Lonza)


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    Structured Review

    Lonza smc maintaining media
    ( a ) SMCs within advanced atherosclerotic lesion specimens were identified based on PLA detection of the <t>SMC</t> specific stable epigenetic signature H3K4dime on the MYH11 . MYH11 H3K4dime PLA + cells exhibit a punctate red dot within the nucleus while the non-nuclear amorphous red staining is autofluorescence or non-specific background. ( a ) Samples were also immuno-stained for CD68 (green), and DAPI (blue). Results showed three distinct cell populations highlighted in enlarged panels to the right and indicated with white arrows: (i) MYH11 H3K4dime PLA + SMCs that are CD68 − , (ii) MYH11 H3K4dime PLA − CD68 + (HSC-derived Mϕs), and (iii) H3K4dime MYH11 H3K4dime PLA + CD68 + SMC-derived Mϕ-like cells. Scale bar = 100 μm. ( b ) Shoulder regions within plaques [stained with DAPI (blue), ACTA2 (green), PLA (red), and CD68 (cyan)] exhibited a high incidence of SMC-derived Mϕ-like cells ( MYH11 H3K4dime PLA + CD68 + ) (yellow arrows) and several phenotypically modulated SMCs negative for CD68 ( MYH11 H3K4dime PLA + ACTA2 − CD68 − ) (white arrows). Scale bar = 50 μm. ( c ) Quantitative analysis of SMC-derived Mϕ-like cells within <t>human</t> <t>coronary</t> lesions based on MYH11 H3K4dime ISH-PLA +/− adjustment for the efficiency of PLA . Error bars = S.E.M. for 12 independent samples of human atherosclerosis in the right coronary artery. ( d ) Combined epigenetic SMC and genetic HSC lineage tracing analyses of cross gender human heart transplant samples. Coronary artery specimens from a male patient who received a female heart were processed for MYH11 H3K4dime PLA (red), Y-chromosome FISH (green), and CD68 staining (yellow). Results show cells that were MYH11 H3K4dime PLA + Y-chromosome − and CD68 + (yellow arrows) reflecting a SMC-derived Mϕ-like cell not of hematopoietic origin (top). In contrast, Mϕs of hematopoietic origin are MYH11 H3K4dime PLA − Y-chromosome + CD68 + (red arrows) (bottom). Scale bar = 50 μm.
    Smc Maintaining Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smc maintaining media/product/Lonza
    Average 90 stars, based on 1 article reviews
    smc maintaining media - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "KLF4 Dependent Phenotypic Modulation of SMCs Plays a Key Role in Atherosclerotic Plaque Pathogenesis"

    Article Title: KLF4 Dependent Phenotypic Modulation of SMCs Plays a Key Role in Atherosclerotic Plaque Pathogenesis

    Journal: Nature medicine

    doi: 10.1038/nm.3866

    ( a ) SMCs within advanced atherosclerotic lesion specimens were identified based on PLA detection of the SMC specific stable epigenetic signature H3K4dime on the MYH11 . MYH11 H3K4dime PLA + cells exhibit a punctate red dot within the nucleus while the non-nuclear amorphous red staining is autofluorescence or non-specific background. ( a ) Samples were also immuno-stained for CD68 (green), and DAPI (blue). Results showed three distinct cell populations highlighted in enlarged panels to the right and indicated with white arrows: (i) MYH11 H3K4dime PLA + SMCs that are CD68 − , (ii) MYH11 H3K4dime PLA − CD68 + (HSC-derived Mϕs), and (iii) H3K4dime MYH11 H3K4dime PLA + CD68 + SMC-derived Mϕ-like cells. Scale bar = 100 μm. ( b ) Shoulder regions within plaques [stained with DAPI (blue), ACTA2 (green), PLA (red), and CD68 (cyan)] exhibited a high incidence of SMC-derived Mϕ-like cells ( MYH11 H3K4dime PLA + CD68 + ) (yellow arrows) and several phenotypically modulated SMCs negative for CD68 ( MYH11 H3K4dime PLA + ACTA2 − CD68 − ) (white arrows). Scale bar = 50 μm. ( c ) Quantitative analysis of SMC-derived Mϕ-like cells within human coronary lesions based on MYH11 H3K4dime ISH-PLA +/− adjustment for the efficiency of PLA . Error bars = S.E.M. for 12 independent samples of human atherosclerosis in the right coronary artery. ( d ) Combined epigenetic SMC and genetic HSC lineage tracing analyses of cross gender human heart transplant samples. Coronary artery specimens from a male patient who received a female heart were processed for MYH11 H3K4dime PLA (red), Y-chromosome FISH (green), and CD68 staining (yellow). Results show cells that were MYH11 H3K4dime PLA + Y-chromosome − and CD68 + (yellow arrows) reflecting a SMC-derived Mϕ-like cell not of hematopoietic origin (top). In contrast, Mϕs of hematopoietic origin are MYH11 H3K4dime PLA − Y-chromosome + CD68 + (red arrows) (bottom). Scale bar = 50 μm.
    Figure Legend Snippet: ( a ) SMCs within advanced atherosclerotic lesion specimens were identified based on PLA detection of the SMC specific stable epigenetic signature H3K4dime on the MYH11 . MYH11 H3K4dime PLA + cells exhibit a punctate red dot within the nucleus while the non-nuclear amorphous red staining is autofluorescence or non-specific background. ( a ) Samples were also immuno-stained for CD68 (green), and DAPI (blue). Results showed three distinct cell populations highlighted in enlarged panels to the right and indicated with white arrows: (i) MYH11 H3K4dime PLA + SMCs that are CD68 − , (ii) MYH11 H3K4dime PLA − CD68 + (HSC-derived Mϕs), and (iii) H3K4dime MYH11 H3K4dime PLA + CD68 + SMC-derived Mϕ-like cells. Scale bar = 100 μm. ( b ) Shoulder regions within plaques [stained with DAPI (blue), ACTA2 (green), PLA (red), and CD68 (cyan)] exhibited a high incidence of SMC-derived Mϕ-like cells ( MYH11 H3K4dime PLA + CD68 + ) (yellow arrows) and several phenotypically modulated SMCs negative for CD68 ( MYH11 H3K4dime PLA + ACTA2 − CD68 − ) (white arrows). Scale bar = 50 μm. ( c ) Quantitative analysis of SMC-derived Mϕ-like cells within human coronary lesions based on MYH11 H3K4dime ISH-PLA +/− adjustment for the efficiency of PLA . Error bars = S.E.M. for 12 independent samples of human atherosclerosis in the right coronary artery. ( d ) Combined epigenetic SMC and genetic HSC lineage tracing analyses of cross gender human heart transplant samples. Coronary artery specimens from a male patient who received a female heart were processed for MYH11 H3K4dime PLA (red), Y-chromosome FISH (green), and CD68 staining (yellow). Results show cells that were MYH11 H3K4dime PLA + Y-chromosome − and CD68 + (yellow arrows) reflecting a SMC-derived Mϕ-like cell not of hematopoietic origin (top). In contrast, Mϕs of hematopoietic origin are MYH11 H3K4dime PLA − Y-chromosome + CD68 + (red arrows) (bottom). Scale bar = 50 μm.

    Techniques Used: Staining, Derivative Assay



    Similar Products

    smc maintaining media  (Lonza)
    90
    Lonza smc maintaining media
    ( a ) SMCs within advanced atherosclerotic lesion specimens were identified based on PLA detection of the <t>SMC</t> specific stable epigenetic signature H3K4dime on the MYH11 . MYH11 H3K4dime PLA + cells exhibit a punctate red dot within the nucleus while the non-nuclear amorphous red staining is autofluorescence or non-specific background. ( a ) Samples were also immuno-stained for CD68 (green), and DAPI (blue). Results showed three distinct cell populations highlighted in enlarged panels to the right and indicated with white arrows: (i) MYH11 H3K4dime PLA + SMCs that are CD68 − , (ii) MYH11 H3K4dime PLA − CD68 + (HSC-derived Mϕs), and (iii) H3K4dime MYH11 H3K4dime PLA + CD68 + SMC-derived Mϕ-like cells. Scale bar = 100 μm. ( b ) Shoulder regions within plaques [stained with DAPI (blue), ACTA2 (green), PLA (red), and CD68 (cyan)] exhibited a high incidence of SMC-derived Mϕ-like cells ( MYH11 H3K4dime PLA + CD68 + ) (yellow arrows) and several phenotypically modulated SMCs negative for CD68 ( MYH11 H3K4dime PLA + ACTA2 − CD68 − ) (white arrows). Scale bar = 50 μm. ( c ) Quantitative analysis of SMC-derived Mϕ-like cells within <t>human</t> <t>coronary</t> lesions based on MYH11 H3K4dime ISH-PLA +/− adjustment for the efficiency of PLA . Error bars = S.E.M. for 12 independent samples of human atherosclerosis in the right coronary artery. ( d ) Combined epigenetic SMC and genetic HSC lineage tracing analyses of cross gender human heart transplant samples. Coronary artery specimens from a male patient who received a female heart were processed for MYH11 H3K4dime PLA (red), Y-chromosome FISH (green), and CD68 staining (yellow). Results show cells that were MYH11 H3K4dime PLA + Y-chromosome − and CD68 + (yellow arrows) reflecting a SMC-derived Mϕ-like cell not of hematopoietic origin (top). In contrast, Mϕs of hematopoietic origin are MYH11 H3K4dime PLA − Y-chromosome + CD68 + (red arrows) (bottom). Scale bar = 50 μm.
    Smc Maintaining Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smc maintaining media/product/Lonza
    Average 90 stars, based on 1 article reviews
    smc maintaining media - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) SMCs within advanced atherosclerotic lesion specimens were identified based on PLA detection of the SMC specific stable epigenetic signature H3K4dime on the MYH11 . MYH11 H3K4dime PLA + cells exhibit a punctate red dot within the nucleus while the non-nuclear amorphous red staining is autofluorescence or non-specific background. ( a ) Samples were also immuno-stained for CD68 (green), and DAPI (blue). Results showed three distinct cell populations highlighted in enlarged panels to the right and indicated with white arrows: (i) MYH11 H3K4dime PLA + SMCs that are CD68 − , (ii) MYH11 H3K4dime PLA − CD68 + (HSC-derived Mϕs), and (iii) H3K4dime MYH11 H3K4dime PLA + CD68 + SMC-derived Mϕ-like cells. Scale bar = 100 μm. ( b ) Shoulder regions within plaques [stained with DAPI (blue), ACTA2 (green), PLA (red), and CD68 (cyan)] exhibited a high incidence of SMC-derived Mϕ-like cells ( MYH11 H3K4dime PLA + CD68 + ) (yellow arrows) and several phenotypically modulated SMCs negative for CD68 ( MYH11 H3K4dime PLA + ACTA2 − CD68 − ) (white arrows). Scale bar = 50 μm. ( c ) Quantitative analysis of SMC-derived Mϕ-like cells within human coronary lesions based on MYH11 H3K4dime ISH-PLA +/− adjustment for the efficiency of PLA . Error bars = S.E.M. for 12 independent samples of human atherosclerosis in the right coronary artery. ( d ) Combined epigenetic SMC and genetic HSC lineage tracing analyses of cross gender human heart transplant samples. Coronary artery specimens from a male patient who received a female heart were processed for MYH11 H3K4dime PLA (red), Y-chromosome FISH (green), and CD68 staining (yellow). Results show cells that were MYH11 H3K4dime PLA + Y-chromosome − and CD68 + (yellow arrows) reflecting a SMC-derived Mϕ-like cell not of hematopoietic origin (top). In contrast, Mϕs of hematopoietic origin are MYH11 H3K4dime PLA − Y-chromosome + CD68 + (red arrows) (bottom). Scale bar = 50 μm.

    Journal: Nature medicine

    Article Title: KLF4 Dependent Phenotypic Modulation of SMCs Plays a Key Role in Atherosclerotic Plaque Pathogenesis

    doi: 10.1038/nm.3866

    Figure Lengend Snippet: ( a ) SMCs within advanced atherosclerotic lesion specimens were identified based on PLA detection of the SMC specific stable epigenetic signature H3K4dime on the MYH11 . MYH11 H3K4dime PLA + cells exhibit a punctate red dot within the nucleus while the non-nuclear amorphous red staining is autofluorescence or non-specific background. ( a ) Samples were also immuno-stained for CD68 (green), and DAPI (blue). Results showed three distinct cell populations highlighted in enlarged panels to the right and indicated with white arrows: (i) MYH11 H3K4dime PLA + SMCs that are CD68 − , (ii) MYH11 H3K4dime PLA − CD68 + (HSC-derived Mϕs), and (iii) H3K4dime MYH11 H3K4dime PLA + CD68 + SMC-derived Mϕ-like cells. Scale bar = 100 μm. ( b ) Shoulder regions within plaques [stained with DAPI (blue), ACTA2 (green), PLA (red), and CD68 (cyan)] exhibited a high incidence of SMC-derived Mϕ-like cells ( MYH11 H3K4dime PLA + CD68 + ) (yellow arrows) and several phenotypically modulated SMCs negative for CD68 ( MYH11 H3K4dime PLA + ACTA2 − CD68 − ) (white arrows). Scale bar = 50 μm. ( c ) Quantitative analysis of SMC-derived Mϕ-like cells within human coronary lesions based on MYH11 H3K4dime ISH-PLA +/− adjustment for the efficiency of PLA . Error bars = S.E.M. for 12 independent samples of human atherosclerosis in the right coronary artery. ( d ) Combined epigenetic SMC and genetic HSC lineage tracing analyses of cross gender human heart transplant samples. Coronary artery specimens from a male patient who received a female heart were processed for MYH11 H3K4dime PLA (red), Y-chromosome FISH (green), and CD68 staining (yellow). Results show cells that were MYH11 H3K4dime PLA + Y-chromosome − and CD68 + (yellow arrows) reflecting a SMC-derived Mϕ-like cell not of hematopoietic origin (top). In contrast, Mϕs of hematopoietic origin are MYH11 H3K4dime PLA − Y-chromosome + CD68 + (red arrows) (bottom). Scale bar = 50 μm.

    Article Snippet: Human coronary SMC were purchased from Lonza and cultured in SMC maintaining media (Lonza).

    Techniques: Staining, Derivative Assay